Prevalence of Trichophyton Species From Selected Soils of Chennai, India

C. Banu Rekha

Abstract


Soil serves as a natural reservoir for both pathogenic and saprophytic fungi. It constitute humus and organic material. More than 100 species of fungi are generally recognized as a pathogen for man found in soil. The majority of dermatophytes can live as saprophytically and  considered as potential pathogen. These fungi can cause different types of tinea in humans. Considering the importance of soil intransmission of diseases, this study aims at assessing the frequency of dermatophyte ie., Trichophyton Species, in various samples collected from school and college playgrounds, public parks, garden, poultry farm, beach, agricultural land, Zoo and from graveyard in Chennai, India. In our present study we isolated Trichophyton Species from selected soils in Chennai. They were isolated by using hair baiting technique. The samples were cultured on Sabouraud's dextrose agar, containing Chloramphenicol and Cyclohexamide, and incubated at 25- 30οC for 3- 4 weeks aerobically. Isolates were identified by colony morphology and microscopically by Lactophenol cotton blue technique technique and also by physiological tests . Eight Species of Trichophyton were identified it includes T.mentagrophytes, T.rubrum, T.terrestre, T.tonsurans, T.verrucosum, T.violaceum, T.equinum, T.concentricum. T.mentagrophytes were found to be higher i.e 60% and followed by T.terrestre to be 44% and followed by T.rubrum found to be 37%. The other isolates are T.verrucosum 17%, T.tonsurans 12%, T.violaceum 08%, T.equinum 03% and least is T.concentricum 02%. Due to the humid and over population in Chennai the pathogenic dermatophyte Trichophyton species are present in all the types of soil and found to more in school and college playground due to the unhygienic status of the students and other reason is open play grounds are often invaded by many animals such as birds, dogs, cats and rats etc. These animals leave organic residue, which probably contaminate the soil which propagules of fungal pathogens.


Keywords


Dermatophytes, Trichophyton, Soil, Hair baiting technique.

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References


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