Degrading of nitrobodies using microorganisms
In this study biodegradation of p-nitrophenol (PNP) by soil microorganisms is evaluated. In biological degradation, performance of pure culture and mixed culture was investigated. It has been observed that the cultivable flora of soil sample was 4x10 7cfu/gm which could utilize PNP 100 ppm as energy source. The organisms were isolated ,identified and screened on the basis of maximum PNP degradation with least time at room temperature. Five bacterial isolates were selected on the basis of their ability to tolerate and degrade PNP 1000ppm. Isolate PD07 could be able to grow and reach absorbance 1 up to within 17 hrs at room temperature. Growth profile of the this isolate showed maximum growth in presence of Nutrient broth and PNP. As minimal media (without nitrogen and carbon source ) was used for whole study it has been concluded that this organism utilizes PNP as carbon and nitrogen source.
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