Preliminary and Secondary Phytochemistry of the Suicidal Plant Cleistanthus collinus (Roxb.)

P Arulmanickam, C Chitra


            The study was planned to identify the primary and secondary phytochemical screenings of Cleistanthus collinus (Roxb.) which is belongs to Euphorbiaceae family. The high polar ability of ethanol was used for extracting  the primary phytochemical compounds from C. collinus leaf, which showed 6 primary phytochemical compounds, such as alkaloids, flavonoids, tannins, polyphenols, saponins and cardiac glycosides, then GC-MS analyses revealed the 13 secondary metabolites. Among them only 7 compounds possessed bioactive properties based on previous literatures out of 13 secondary metabolites. So, C. collinus has important amount of primary and secondary phytochemicals properties.


Cleistanthus collinus, Euphorbiaceae, Phytochemicals, GC-MS analyses, Ethanolic extracts

Full Text:



Ahirwar, B., Ahirwar, D., & Lanjhiayana, S. (2011). Insecticidal activity of Cleistanthus collinus Roxb. Der Pharma Chemica, 3(1), 49-52.

Anapoorani, K.S. (1985). Analytical biochemistry in forensic sciences: Toxicological aspects of Cleistanthus collinus. A PhD thesis submitted to the University of Madras.

Annapoorani, K.S., Damodaran, C., & Chabdrasekharan, P.A. (1986). Promising Antidote to Cleistanthus collinus poisoning. J forensic Sci, 2, 3-6.

Annapoorani, K.S., Periakali, P., Illangovan, S., Damodaran, C., & Chabdrasekharan, P. (1984). Spectrofluorometric Determination of the Toxic constituents of Cleistanthus collinus. J Anal Toxicol, 8,182-6

Asase A., Akwetey G.A., & Achel D.G., (2010). Ethnopharmacological use of herbal remedies for the treatment of malaria in the Dangme West District of Ghana. J. Ethnopharmacol, 129, 367-376.

Bergh, J. C., Lazovios, A., Somogyi, G., Lengyel, L., & Feher, J. (2006). "The first clinical pilot of Roquinimax (Linomide) fl cancer patients with special focus on immunological effects," Cancer Investigation, 15, 204-211.

Boots, A.W., Haenen, G.R., & Bast, A.( 2008). Health effects of quercetin: From antioxidant to nutraceutical. Eur. J. Pharmacol, 585, 325–337.

Charnley G, Omenn GS (1997) A summary of the findings and recommendations of the commission on risk assessment and risk management of herbal drugs. Hum Ecol Risk Assess, 3, 701-711.

Eswarappa, S., Chakraborty, A.R., Palatty, B.U., & Vasnaik, M. (2003). Cleistanthus collinus poisoning: case report and review of the literature. J Toxicol,41, 369-72.

Heide, L., & Bogi, V. (1986). "The identification of irradiated dried food stuffs by luminescence measurement," Food Lab Newsletter, 5, 12-23.

Kabir, A.K.M.S., Matin, M.M., Bhuiyan, M.M.R., Rahim, M.A., & Rahman, M.S. (2005). Biological evaluation of some monosaccharide derivatives. Int. J. Agric. Biol., 7, 218-221.

Kim Y.C., Kim H., Wataya Y., Sohn D.H., Kang T.H., Kim M.S., Kim Y.M., Lee G.M., Chang, J.D., & Park, H. (2004). Antimalarial Activity of Lavandulyl Flavanones Isolated from the Roots of Sophora flavescens. Biol. Pharm. Bull., 27, 748-750.

Kokate, K. K., Purohit, A. P., & Gokhale, S. B. (2008). Pharmacognosy, 42nd ed. India: Vallabh Prakashan.

Nandakumar, N.V., Pagala, M.K.D., Venkataciiari, S.A.T., Namba, T., & Grob, D. (1989). Effect of Cleistanthus collinus leaf extract on neuromuscular function of the isolated mouse phrenic nerve-diaphragm. Toxicon, 27, 1219-28.

Prabhadevi, V., SahayaSathish, S., Johnson, M., Venkatramani, B., & Janakiraman, N. (2012). Phytochemical studies on Allamanda cathartica L. using GC-MS. Asian Pac J Trop Med.,S550-S554.

Philipson J.D., Wright C.W. (1991). Antiprotozoal compounds from plants sources. Planta Med., 57, 553-559.

Rabi, T., & Bishayee, A. (2009). "Terpenoids and breast cancer chemoprevention," Breast Cancer Research and Treatment, vol. 155, 223-233.

Rao, A. V., & Gurfinkel, D. M., (2000). "The bioactivity of saponins: triterpenoid and steroidal glycosides," Drug Metabolism and Drug Interactions, 17, 211-236.

Revathi, P., Parimelazhagan, T., & Manian, S. (2013)."Quantification of phenolic compounds, In vitro antioxidant analysis and screening of chemical compounds using GC-MS in Acalypha alnifolia Klein Ex wild. - a leafy vegetable," International Journal of Pharma and Bio Sciences, 4, 973-986.

Selvamuthukumaran, T. & Arivudainambi. S. 2008b. Insecticidal properties of Cleistanthus collinus (Roxb.) Benth (Family: Euphorbiaceae) against Spodoptera litura Fab. (Noctuidae: Lepidoptera). Plant Archives, 8(2): 683 – 685.

Sharma, D. K. (2006). "Pharmacological properties of flavonoids including flavonolignans-integration of petrocrops with drug development from plants," Journal of Scientific and Industrial Research, 65, 477-484.

Shulka, Y. M., Dhruve, J. J., Patel, N. J., Bhatnagar, R., Talati, J. G., & Kathiria, K. B. ((2009). Plant Secondary Meatabolites. New Delhi, India: New India Publishing Agency.

Subramanian, R., & Krishnamurthy, G. (1975). Thin-layer chromatographic detection of the lignin lactones of Cleistanthus collinus (Roxb.). J Chromatogr,107, 230-3.

Suffredini, I.B., Sader, H.S., Gonçalves, A.G., Reis, A.O., Gales, A.C., Varella, A.D., & Younes, R.N. (2004).Screening of antibacterial extracts from plants native to the brazilian amazon rain forest and atlantic forest. Braz. J. Med. Biol. Res, 37, 379–384.

Sumathi, C.S., Balasubramaniyan, V., Ramesh, N.A., & Kannan, R. (2008). Influence of Biotic and Abiotic features on Curcuma longa L. Plantation under Tropical condition. Middle East J. Sci, 3, 171- 178.

Tasdemir, D., Kaiser, D., Brun, R., Yardley, V., Schmidt, T.J., Tosun, F., & Ruedi. P. (2006) Antitrypanosomal and antileishmanial activities of flavonoids and their analogues: In vitro, in vivo, structure-activity relationship, and quantitative structure-activity relationship studies. Antimicrob. Agents Chemother. 50, 1352-1364

Trease, G. E., & Evans, W. C. (1978). Pharmacology, 11th ed. London: Bailliere Tindall Ltd., [13] H.

Tripathi, K. D. (2004). Cardiac glycosides and drugs for CCF, Essentials of medical pharmacology, 5th ed. Medical Publishers.

Van Rooyen, N., & Theron, G.K. (1996). Habitat Evaluation, In: Bothma, J. du P. Game Ranch Management , Van Schaik Publishers, Pretoria., 70-73.

Wu, H., Haig, T., Pratley, J., Lemerie, D., & An, M. (2000). "Allelochemicals in wheat (Triticum aestivum L.), variation of phenolic acids in root tissue," Journal of Agricultural and Food Chemistry, 48, 5321-5325.

Zhang, Z.J., Tan, Q.R., Tong, Y., Wang, X.Y., & Wang, H.H. et al. (2011). An epidemiological study of concomitant use of Chinese medicine and Toxic constituents of the leaf are Arylnapthalideligans- Cleistanthin A and B’: implication for herb-drug interaction. PLoS ONE 6: 2.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.